Proteomics Provide Insight into the Interaction between Selenite and the Microalgae Dunaliella salina

Dunaliella salina is currently one of the most commercially valuable microalgae species in world. In reponse to selenite, D. a with high selenium content, thereby increasing its value, which crucial for economic value as nutrional supplement. However, effects selenite on are still unclear, and molecular mechanism response stress also elusive. Here, order study corresponding regulatory mechanism, we characterized physiological phenotypes under different concentrations carried out quantitative proteomic study. The results showed that effective concentration 50% growth inhibition (EC50) algae was 192.7 mg/L after 11 days cultivation. When lower than 100 mg/L, did not hinder early stage, but shortened cell cycle, although significantly inhibited when higher 250 mg/L. Bioaccumulation experiments content intracellular cells reached highest level treatment 50 contents total organic were 499.77 ?g/g 303.01 (dry weight), respectively. Proteomic analysis revealed series proteins related responses, amino acid metabolism energy production pathways profoundly altered by treatment. Glutathione peroxidase (GPX7), selenium-containing protein, identified group given Moreover, involved photoreactions oxidative phosphorylation upregulated, indicating effectively balanced demand stress. This provides novel insights into responses salina, candidate biological carrier would be helpful development industrial strains rich selenium.